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Nanoparticle Surface Protein Analysis

NanoFCM

Characterize nanoparticles and extracellular vesicles (EVs) from almost any fluid and biofluid - from plasma to tissue culture media

Nano-flow cytometry is the latest development for protein analysis on nanoparticles. Using light scattering and labelled fluorescent antibodies, our analysis allows direct, single-particle physical and bio-chemical measurements of nano-sized entities on a single platform.
奈米表面蛋白分析 NanoFCM | Reliance Biosciences | Molecular diagnostics via exosome
奈米表面蛋白分析 NanoFCM Size Distribution_Reliance Biosciences_Molecular diagnostics via exosome

Fig. 1. Detectable range of EVs
(30nm – 2000nm)

NanoFCM Case Study

Using fluorescence and measurements of particle size and concentration to determine breast cancer HER2 status

HER2 is a growth-promoting protein found on 15-20% of breast cancer cells. These cells tend to grow and spread faster than normal breast cells. In the following analysis, HER2 antibodies were labeled exosomes from MCF7 cell line (HER2-Negative), 82T primary cell line (HER2-Positive metastatic site), AU565 cell line (HER2-Positive), and healthy donor (HD), were tested with PBS (blank) as control. The figure below shows HER2-Positive signals above the cut-off for 82T and AU565. The other signals below cut-off indicate HER2-Negative.
Characteriszing exosomes nanofcm

Publication Library

Read about Nano-flow cytometry (NanoFCM) applications in industry and other research uses in these published papers.

Sample Reports

Sample Preparation Instructions

FAQs

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